Isolation and structure of a brain constituent that binds to the cannabinoid receptor

Citation metadata

Authors: William A. Devane, Lumir Hanus, Aviva Breuer, Roger G. Pertwee, Lesley A. Stevenson and Graeme Griffin
Date: Dec. 18, 1992
From: Science(Vol. 258, Issue 5090)
Publisher: American Association for the Advancement of Science
Document Type: Article
Length: 2,639 words

Main content

Abstract :

Arachidonylethanolamide, an arachidonic acid derivative in porcine brain, was identified in a screen for endogenous ligands for the cannabinoid receptor. The structure of this compound, which has been named "anandamide," was determined by mass spectrometry and nuclear magnetic resonance spectroscopy and was confirmed by synthesis. Anandamide inhibited the specific binding of a radiolabeled cannabinoid probe to synaptosomal membranes in a manner typical of competitive ligands and produced a concentration-dependent inhibition of the electrically evoked twitch response of the mouse vas deferens, a characteristic effect of psychotropic cannabinoids. These properties suggest that anandamide may function as a natural ligand for the cannabinoid receptor.

Source Citation

Source Citation
Devane, William A., et al. "Isolation and structure of a brain constituent that binds to the cannabinoid receptor." Science, vol. 258, no. 5090, 18 Dec. 1992, pp. 1946+. Accessed 4 Dec. 2022.

Gale Document Number: GALE|A13320461